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pCC1BAC.gb
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pCC1BAC.gb
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LOCUS Exported 8139 bp DNA circular SYN 29-AUG-2024
DEFINITION Cloning vector pCC1BAC, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8139)
AUTHORS EPICENTRE Biotechnologies.
TITLE Direct Submission
JOURNAL Submitted (23-AUG-2007) 726 Post Road, Madison, WI 53713, USA
REFERENCE 2 (bases 1 to 8139)
TITLE Direct Submission
REFERENCE 3 (bases 1 to 8139)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 8139)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
(23-AUG-2007) 726 Post Road, Madison, WI 53713, USA"
COMMENT SGRef: number: 2; type: "Journal Article"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..8139
/mol_type="other DNA"
/organism="synthetic DNA construct"
source 359..370
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 288..304
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 311..329
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 332..400
/label=MCS
/note="pUC18/19 multiple cloning site"
misc_feature 359..370
/label=MCS
/note="pUC18/19 multiple cloning site"
primer_bind complement(443..459)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(467..483)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(491..521)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(536..557)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
CDS complement(780..1436)
/codon_start=1
/label=CmR
/note="chloramphenicol acetyltransferase"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
promoter complement(1437..1539)
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
rep_origin 2397..3011
/label=oriV
/note="origin of replication for the bacterial F plasmid"
rep_origin 3087..3306
/label=ori2
/note="secondary origin of replication for the bacterial F
plasmid; also known as oriS"
CDS 3397..4149
/codon_start=1
/label=repE
/note="replication initiation protein for the bacterial F
plasmid"
/translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
misc_feature 4155..4405
/label=incC
/note="incompatibility region of the bacterial F plasmid"
CDS 4731..5903
/codon_start=1
/label=sopA
/note="partitioning protein for the bacterial F plasmid"
/translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
CDS 5906..6874
/codon_start=1
/label=sopB
/note="partitioning protein for the bacterial F plasmid"
/translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
misc_feature 6950..7423
/label=sopC
/note="centromere-like partitioning region of the bacterial
F plasmid"
misc_feature complement(7683..8081)
/label=cos
/note="lambda cos site; allows packaging into phage lambda
particles"
protein_bind complement(8099..8132)
/label=loxP
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
1 gcggccgcaa ggggttcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
61 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
121 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt cagctgcgca actgttggga
181 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
241 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
301 cagtgaattg taatacgact cactataggg cgaattcgag ctcggtaccc ggggatccca
361 cgtgggatcc tctagagtcg acctgcaggc atgcaagctt gagtattcta tagtctcacc
421 taaatagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
481 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
541 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
601 cgtgccagct gcattaatga atcggccaac gcgaacccct tgcggccgcc cgggccgtcg
661 accaattctc atgtttgaca gcttatcatc gaatttctgc cattcatccg cttattatca
721 cttattcagg cgtagcaacc aggcgtttaa gggcaccaat aactgcctta aaaaaattac
781 gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg
841 aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct
901 tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg
961 tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca
1021 ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat
1081 atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca
1141 gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg
1201 tctttcattg ccatacgaaa ttccggatga gcattcatca ggcgggcaag aatgtgaata
1261 aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc
1321 agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct
1381 ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta
1441 gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt
1501 tcattatggt gaaagttgga acctcttacg tgccgatcaa cgtctcattt tcgccaaaag
1561 ttggcccagg gcttcccggt atcaacaggg acaccaggat ttatttattc tgcgaagtga
1621 tcttccgtca caggtattta ttcgcgataa gctcatggag cggcgtaacc gtcgcacagg
1681 aaggacagag aaagcgcgga tctgggaagt gacggacaga acggtcagga cctggattgg
1741 ggaggcggtt gccgccgctg ctgctgacgg tgtgacgttc tctgttccgg tcacaccaca
1801 tacgttccgc cattcctatg cgatgcacat gctgtatgcc ggtataccgc tgaaagttct
1861 gcaaagcctg atgggacata agtccatcag ttcaacggaa gtctacacga aggtttttgc
1921 gctggatgtg gctgcccggc accgggtgca gtttgcgatg ccggagtctg atgcggttgc
1981 gatgctgaaa caattatcct gagaataaat gccttggcct ttatatggaa atgtggaact
2041 gagtggatat gctgtttttg tctgttaaac agagaagctg gctgttatcc actgagaagc
2101 gaacgaaaca gtcgggaaaa tctcccatta tcgtagagat ccgcattatt aatctcagga
2161 gcctgtgtag cgtttatagg aagtagtgtt ctgtcatgat gcctgcaagc ggtaacgaaa
2221 acgatttgaa tatgccttca ggaacaatag aaatcttcgt gcggtgttac gttgaagtgg
2281 agcggattat gtcagcaatg gacagaacaa cctaatgaac acagaaccat gatgtggtct
2341 gtccttttac agccagtagt gctcgccgca gtcgagcgac agggcgaagc cctcggctgg
2401 ttgccctcgc cgctgggctg gcggccgtct atggccctgc aaacgcgcca gaaacgccgt
2461 cgaagccgtg tgcgagacac cgcggccggc cgccggcgtt gtggatacct cgcggaaaac
2521 ttggccctca ctgacagatg aggggcggac gttgacactt gaggggccga ctcacccggc
2581 gcggcgttga cagatgaggg gcaggctcga tttcggccgg cgacgtggag ctggccagcc
2641 tcgcaaatcg gcgaaaacgc ctgattttac gcgagtttcc cacagatgat gtggacaagc
2701 ctggggataa gtgccctgcg gtattgacac ttgaggggcg cgactactga cagatgaggg
2761 gcgcgatcct tgacacttga ggggcagagt gctgacagat gaggggcgca cctattgaca
2821 tttgaggggc tgtccacagg cagaaaatcc agcatttgca agggtttccg cccgtttttc
2881 ggccaccgct aacctgtctt ttaacctgct tttaaaccaa tatttataaa ccttgttttt
2941 aaccagggct gcgccctgtg cgcgtgaccg cgcacgccga aggggggtgc ccccccttct
3001 cgaaccctcc cggtcgagtg agcgaggaag caccagggaa cagcacttat atattctgct
3061 tacacacgat gcctgaaaaa acttcccttg gggttatcca cttatccacg gggatatttt
3121 tataattatt ttttttatag tttttagatc ttctttttta gagcgccttg taggccttta
3181 tccatgctgg ttctagagaa ggtgttgtga caaattgccc tttcagtgtg acaaatcacc
3241 ctcaaatgac agtcctgtct gtgacaaatt gcccttaacc ctgtgacaaa ttgccctcag
3301 aagaagctgt tttttcacaa agttatccct gcttattgac tcttttttat ttagtgtgac
3361 aatctaaaaa cttgtcacac ttcacatgga tctgtcatgg cggaaacagc ggttatcaat
3421 cacaagaaac gtaaaaatag cccgcgaatc gtccagtcaa acgacctcac tgaggcggca
3481 tatagtctct cccgggatca aaaacgtatg ctgtatctgt tcgttgacca gatcagaaaa
3541 tctgatggca ccctacagga acatgacggt atctgcgaga tccatgttgc taaatatgct
3601 gaaatattcg gattgacctc tgcggaagcc agtaaggata tacggcaggc attgaagagt
3661 ttcgcgggga aggaagtggt tttttatcgc cctgaagagg atgccggcga tgaaaaaggc
3721 tatgaatctt ttccttggtt tatcaaacgt gcgcacagtc catccagagg gctttacagt
3781 gtacatatca acccatatct cattcccttc tttatcgggt tacagaaccg gtttacgcag
3841 tttcggctta gtgaaacaaa agaaatcacc aatccgtatg ccatgcgttt atacgaatcc
3901 ctgtgtcagt atcgtaagcc ggatggctca ggcatcgtct ctctgaaaat cgactggatc
3961 atagagcgtt accagctgcc tcaaagttac cagcgtatgc ctgacttccg ccgccgcttc
4021 ctgcaggtct gtgttaatga gatcaacagc agaactccaa tgcgcctctc atacattgag
4081 aaaaagaaag gccgccagac gactcatatc gtattttcct tccgcgatat cacttccatg
4141 acgacaggat agtctgaggg ttatctgtca cagatttgag ggtggttcgt cacatttgtt
4201 ctgacctact gagggtaatt tgtcacagtt ttgctgtttc cttcagcctg catggatttt
4261 ctcatacttt ttgaactgta atttttaagg aagccaaatt tgagggcagt ttgtcacagt
4321 tgatttcctt ctctttccct tcgtcatgtg acctgatatc gggggttagt tcgtcatcat
4381 tgatgagggt tgattatcac agtttattac tctgaattgg ctatccgcgt gtgtacctct
4441 acctggagtt tttcccacgg tggatatttc ttcttgcgct gagcgtaaga gctatctgac
4501 agaacagttc ttctttgctt cctcgccagt tcgctcgcta tgctcggtta cacggctgcg
4561 gcgagcgcta gtgataataa gtgactgagg tatgtgctct tcttatctcc ttttgtagtg
4621 ttgctcttat tttaaacaac tttgcggttt tttgatgact ttgcgatttt gttgttgctt
4681 tgcagtaaat tgcaagattt aataaaaaaa cgcaaagcaa tgattaaagg atgttcagaa
4741 tgaaactcat ggaaacactt aaccagtgca taaacgctgg tcatgaaatg acgaaggcta
4801 tcgccattgc acagtttaat gatgacagcc cggaagcgag gaaaataacc cggcgctgga
4861 gaataggtga agcagcggat ttagttgggg tttcttctca ggctatcaga gatgccgaga
4921 aagcagggcg actaccgcac ccggatatgg aaattcgagg acgggttgag caacgtgttg
4981 gttatacaat tgaacaaatt aatcatatgc gtgatgtgtt tggtacgcga ttgcgacgtg
5041 ctgaagacgt atttccaccg gtgatcgggg ttgctgccca taaaggtggc gtttacaaaa
5101 cctcagtttc tgttcatctt gctcaggatc tggctctgaa ggggctacgt gttttgctcg
5161 tggaaggtaa cgacccccag ggaacagcct caatgtatca cggatgggta ccagatcttc
5221 atattcatgc agaagacact ctcctgcctt tctatcttgg ggaaaaggac gatgtcactt
5281 atgcaataaa gcccacttgc tggccggggc ttgacattat tccttcctgt ctggctctgc
5341 accgtattga aactgagtta atgggcaaat ttgatgaagg taaactgccc accgatccac
5401 acctgatgct ccgactggcc attgaaactg ttgctcatga ctatgatgtc atagttattg
5461 acagcgcgcc taacctgggt atcggcacga ttaatgtcgt atgtgctgct gatgtgctga
5521 ttgttcccac gcctgctgag ttgtttgact acacctccgc actgcagttt ttcgatatgc
5581 ttcgtgatct gctcaagaac gttgatctta aagggttcga gcctgatgta cgtattttgc
5641 ttaccaaata cagcaatagt aatggctctc agtccccgtg gatggaggag caaattcggg
5701 atgcctgggg aagcatggtt ctaaaaaatg ttgtacgtga aacggatgaa gttggtaaag
5761 gtcagatccg gatgagaact gtttttgaac aggccattga tcaacgctct tcaactggtg
5821 cctggagaaa tgctctttct atttgggaac ctgtctgcaa tgaaattttc gatcgtctga
5881 ttaaaccacg ctgggagatt agataatgaa gcgtgcgcct gttattccaa aacatacgct
5941 caatactcaa ccggttgaag atacttcgtt atcgacacca gctgccccga tggtggattc
6001 gttaattgcg cgcgtaggag taatggctcg cggtaatgcc attactttgc ctgtatgtgg
6061 tcgggatgtg aagtttactc ttgaagtgct ccggggtgat agtgttgaga agacctctcg
6121 ggtatggtca ggtaatgaac gtgaccagga gctgcttact gaggacgcac tggatgatct
6181 catcccttct tttctactga ctggtcaaca gacaccggcg ttcggtcgaa gagtatctgg
6241 tgtcatagaa attgccgatg ggagtcgccg tcgtaaagct gctgcactta ccgaaagtga
6301 ttatcgtgtt ctggttggcg agctggatga tgagcagatg gctgcattat ccagattggg
6361 taacgattat cgcccaacaa gtgcttatga acgtggtcag cgttatgcaa gccgattgca
6421 gaatgaattt gctggaaata tttctgcgct ggctgatgcg gaaaatattt cacgtaagat
6481 tattacccgc tgtatcaaca ccgccaaatt gcctaaatca gttgttgctc ttttttctca
6541 ccccggtgaa ctatctgccc ggtcaggtga tgcacttcaa aaagccttta cagataaaga
6601 ggaattactt aagcagcagg catctaacct tcatgagcag aaaaaagctg gggtgatatt
6661 tgaagctgaa gaagttatca ctcttttaac ttctgtgctt aaaacgtcat ctgcatcaag
6721 aactagttta agctcacgac atcagtttgc tcctggagcg acagtattgt ataagggcga
6781 taaaatggtg cttaacctgg acaggtctcg tgttccaact gagtgtatag agaaaattga
6841 ggccattctt aaggaacttg aaaagccagc accctgatgc gaccacgttt tagtctacgt
6901 ttatctgtct ttacttaatg tcctttgtta caggccagaa agcataactg gcctgaatat
6961 tctctctggg cccactgttc cacttgtatc gtcggtctga taatcagact gggaccacgg
7021 tcccactcgt atcgtcggtc tgattattag tctgggacca cggtcccact cgtatcgtcg
7081 gtctgattat tagtctggga ccacggtccc actcgtatcg tcggtctgat aatcagactg
7141 ggaccacggt cccactcgta tcgtcggtct gattattagt ctgggaccat ggtcccactc
7201 gtatcgtcgg tctgattatt agtctgggac cacggtccca ctcgtatcgt cggtctgatt
7261 attagtctgg aaccacggtc ccactcgtat cgtcggtctg attattagtc tgggaccacg
7321 gtcccactcg tatcgtcggt ctgattatta gtctgggacc acgatcccac tcgtgttgtc
7381 ggtctgatta tcggtctggg accacggtcc cacttgtatt gtcgatcaga ctatcagcgt
7441 gagactacga ttccatcaat gcctgtcaag ggcaagtatt gacatgtcgt cgtaacctgt
7501 agaacggagt aacctcggtg tgcggttgta tgcctgctgt ggattgctgc tgtgtcctgc
7561 ttatccacaa cattttgcgc acggttatgt ggacaaaata cctggttacc caggccgtgc
7621 cggcacgtta accgggctgc atccgatgca agtgtgtcgc tgtcgacgag ctcgcgagct
7681 cggacatgag gttgccccgt attcagtgtc gctgatttgt attgtctgaa gttgttttta
7741 cgttaagttg atgcagatca attaatacga tacctgcgtc ataattgatt atttgacgtg
7801 gtttgatggc ctccacgcac gttgtgatat gtagatgata atcattatca ctttacgggt
7861 cctttccggt gatccgacag gttacggggc ggcgacctcg cgggttttcg ctatttatga
7921 aaattttccg gtttaaggcg tttccgttct tcttcgtcat aacttaatgt ttttatttaa
7981 aataccctct gaaaagaaag gaaacgacag gtgctgaaag cgagcttttt ggcctctgtc
8041 gtttcctttc tctgtttttg tccgtggaat gaacaatgga agtccgagct catcgctaat
8101 aacttcgtat agcatacatt atacgaagtt atattcgat
//