Error getting response from yubikey

[mercury1986]

HI
I have the following issue:
usb-stick with keepass2.41 portable + keechallenge plugin
HMAC SHA1 on slot 1 (Fixed 64 byte input and require user input)
yubikey 5 NFC
keepass.kdbx with masterkey and keyfile: "Yubikey challenge-response"

PC1 win10 1809:
insert usb stick --> open keepass.kdbx --> enter masterkey --> press yubikey --> keepass.kdbx opened

PC2 win10 1803:
insert same usb-stick --> open keepass.kdbx --> enter masterkey --> Error "Error getting response from yubikey" --> can not open keepass.kdbx

On PC2:
I try ykchalresp -1 -t and get an response
I try yubico demo auth website --> everthing works

Can you help me to solve the issue?

[synologic]

Seems like a long time to go without an answer :)
I have the same issue though ...

[eduardable]

This issue occurs when you have the challenge response on slot 1, but what happens in KeePass is, the slot resets. So if you selected slot 1 initially, when you restart, it gets reset to slot 2. You can change this simply by going to Tools -> KeeChallenge Settings -> Slot 1, then it should work again.

Obviously my response is a bit late for you two, but I hope it might help people in the future.