Skip to content

Commit

Permalink
Update FFPred_README
Browse files Browse the repository at this point in the history
Made some updates to the install instructions
  • Loading branch information
DanBuchan authored May 1, 2019
1 parent d014256 commit 2ee8a40
Showing 1 changed file with 10 additions and 7 deletions.
17 changes: 10 additions & 7 deletions FFPred_README
Original file line number Diff line number Diff line change
Expand Up @@ -18,8 +18,7 @@
pieces of third-party software. This code is capable of running analyses with
Human SVMs v3.0 and Fly SVMs v1.0 (see below)
Detailed instructions on how to set everything up are reported below. Please
take some time to read them carefully and in sequential order - ignore them
at your peril!
take some time to read them carefully and in sequential order

#------------------------------------------------------------------------------

Expand Down Expand Up @@ -70,8 +69,9 @@ ITS USE AND ANY RESULTS OBTAINED.
SIGNALP http://www.cbs.dtu.dk/cgi-bin/nph-sw_request?signalp
WoLFPSORT http://bioinfadmin.cs.ucl.ac.uk/downloads/ffpred_feature_suite/wolfpsort_0.2.zip
PFILT http://bioinfadmin.cs.ucl.ac.uk/downloads/pfilt/
BLAST ftp://ftp.ncbi.nih.gov/blast/executables/
BLAST ftp://ftp.ncbi.nlm.nih.gov/blast/executables/legacy.NOTSUPPORTED/2.2.26/
SVM-Light http://svmlight.joachims.org/
Uniref90 ftp://ftp.uniprot.org/pub/databases/uniprot/uniref/uniref90/uniref90.fasta.gz

Perl 5.6 or later, including core modules Getopt::Long and Digest::MD5

Expand All @@ -95,10 +95,10 @@ ITS USE AND ANY RESULTS OBTAINED.

chmod a+x FFPred.pl
chmod a+x featurama/features.pl
chmod a+x material/uniref/UniRef_xml_to_fasta.pl

Also, make sure the relevant folders can be written to:

mkdir bin data featurama SVMs uniref90
chmod -R u+w bin data featurama SVMs uniref90
chmod a+w jobs
chmod a+w out
Expand All @@ -113,8 +113,8 @@ ITS USE AND ANY RESULTS OBTAINED.
perl -c FFPred.pl
perl -c featurama/features.pl

Finally, note that the release includes a configuration file, called
"FFPred4/CONFIG", that can be edited to set many useful paths (all of them
Finally, edit the included a configuration file, called
"FFPred4/CONFIG", to set the paths (all of them
are explained in detail in what follows):

PFILT directory containing the executable for pfilt software
Expand All @@ -134,7 +134,7 @@ ITS USE AND ANY RESULTS OBTAINED.
SEQFEAT directory containing the executable for SeqFeat
PEST directory containing the executable for EMBOSS/pestefind software
PATH directory for temporary FFPred4 files
DB full path to FASTA database (UniRef90)
DB full path to FASTA database (UniRef90)
MASK_DB full path to masked version of FASTA database (UniRef90)

Importantly, as explained in the file header, all paths need to be specified
Expand All @@ -147,6 +147,9 @@ ITS USE AND ANY RESULTS OBTAINED.
IMPORTANT: You should ensure each of these packages is installed and working
correctly before you attempt to run FFPred. NETPHOS, NETOGLYC, NETNGLYC will
all need to be hand edited.

IMPORTANT: You must ensure that the correct blast database created using the
BLAST formatdb tool

#------------------------------------------------------------------------------

Expand Down

0 comments on commit 2ee8a40

Please sign in to comment.