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No newick file #629

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been9312 opened this issue Nov 18, 2024 · 0 comments
Open

No newick file #629

been9312 opened this issue Nov 18, 2024 · 0 comments

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@been9312
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I ran roary with two gff files but, I can't get accessory_binary_genes.fa.newick file with messages below.

@DESKTOP-G3IK25V:/mnt/d/GAS/FastQ/concen/final/12s$ roary -e --mafft -p 8 12.gff 12-2.gff

------------- EXCEPTION: Bio::Root::Exception -------------
MSG: Could not read file 'pan_genome_sequences/zosA.fa.aln': No such file or directory
STACK: Error::throw
STACK: Bio::Root::Root::throw /usr/share/perl5/Bio/Root/Root.pm:449
STACK: Bio::Root::IO::_initialize_io /usr/share/perl5/Bio/Root/IO.pm:272
STACK: Bio::SeqIO::_initialize /usr/share/perl5/Bio/SeqIO.pm:508
STACK: Bio::SeqIO::fasta::_initialize /usr/share/perl5/Bio/SeqIO/fasta.pm:88
STACK: Bio::SeqIO::new /usr/share/perl5/Bio/SeqIO.pm:384
STACK: Bio::SeqIO::new /usr/share/perl5/Bio/SeqIO.pm:430
STACK: Bio::Roary::SortFasta::_input_seqio /usr/share/perl5/Bio/Roary/SortFasta.pm:27
STACK: Bio::Roary::SortFasta::sort_fasta /usr/share/perl5/Bio/Roary/SortFasta.pm:68
STACK: Bio::Roary::CommandLine::GeneAlignmentFromNucleotides::run /usr/share/perl5/Bio/Roary/CommandLine/GeneAlignmentFromNucleotides.pm:107
STACK: /usr/bin/protein_alignment_from_nucleotides:14

Use of uninitialized value $gene_length in addition (+) at /usr/share/perl5/Bio/Roary/Output/CoreGeneAlignmentCoordinatesEMBL.pm line 36.
Use of uninitialized value $gene_length in addition (+) at /usr/share/perl5/Bio/Roary/Output/CoreGeneAlignmentCoordinatesEMBL.pm line 36.
Use of uninitialized value $gene_length in addition (+) at /usr/share/perl5/Bio/Roary/Output/CoreGeneAlignmentCoordinatesEMBL.pm line 36.
Use of uninitialized value $gene_length in addition (+) at /usr/share/perl5/Bio/Roary/Output/CoreGeneAlignmentCoordinatesEMBL.pm line 36.

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