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Can't open file: proteome.faa #631

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alanxelena opened this issue Dec 9, 2024 · 0 comments
Open

Can't open file: proteome.faa #631

alanxelena opened this issue Dec 9, 2024 · 0 comments

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@alanxelena
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Dear Sanger team,

I'm having an issue running roary. I've run the following command: roary -p 20 -f ./Results *.gff. The Results directory together with the gff files gets created but I get the following error:

Cant open file: /home/service/Results/0G3_sO09fo/DNA1.gff.proteome.faa

I tried adding -z to the command to keep the temporary files but that doesn't help. Actually the file DNA1.gff.proteome.faa is not to be found in the 0G3_sO09fo directory but rather DNA1.gff.proteome.faa.tmp. This file is a protein multifasta.

I've also run roary -a and the only warning I get is

Looking for 'cd-hit' - found
Use of uninitialized value in concatenation (.) or string at /usr/perl5/Bio/Roary/External/CheckTools.pm line 131.
Determined cd-hit version is Use of uninitialized value in numeric lt (<) at /usr/perl5/Bio/Roary/External/CheckTools.pm line 132.
Roary needs cd-hit 4.6 or higher. Please upgrade and try again.

Still, I have cd-hit v4.8.1.

Any help on this issue would be really appreciated.

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