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Merge pull request #5 from Kincekara/1.4.0-dev
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1.4.0
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Kincekara authored Jan 26, 2024
2 parents e2154df + 9d2e28d commit 916f3e3
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10 changes: 5 additions & 5 deletions README.md
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Expand Up @@ -35,16 +35,16 @@ The following programs and tools are used in the C-BIRD pipeline.
| Tools | Version | Comments |
| --- | --- | --- |
| [FastP](https://github.com/OpenGene/fastp) | 0.23.4 | QC, adapter removal, quality filtering and trimming |
| [BBTools](https://jgi.doe.gov/data-and-tools/software-tools/bbtools/) | 39.01 | phiX removal & optional normalization |
| [BBTools](https://jgi.doe.gov/data-and-tools/software-tools/bbtools/) | 39.06 | phiX removal & optional normalization |
| [Kraken2](https://github.com/DerrickWood/kraken2) | 2.1.3 | Taxonomic profiling & contamination check |
| [Bracken](https://github.com/jenniferlu717/Bracken) | 2.9 | Abundance estimation |
| [SPAdes](https://github.com/ablab/spades) | 3.15.5 | *De novo* assembly |
| [Mash](https://github.com/marbl/Mash) | 2.3 | Bacterial identification |
| [QUAST](https://github.com/ablab/quast) | 5.2.0 | Genome assembly evaluation |
| [BUSCO](https://gitlab.com/ezlab/busco/-/tree/master) | 5.4.7 | Genomic data quality assesment |
| [mlst](https://github.com/tseemann/mlst) | 2.22.0 | MLST typing |
| [AMRFinderPlus](https://github.com/ncbi/amr) | 3.11.20 | AMR gene identification |
| [BLAST+](https://blast.ncbi.nlm.nih.gov/doc/blast-help/downloadblastdata.html)| 2.14.0 | Target gene search |
| [BUSCO](https://gitlab.com/ezlab/busco/-/tree/master) | 5.6.1 | Genomic data quality assesment |
| [mlst](https://github.com/tseemann/mlst) | 2.23.0 | MLST typing |
| [AMRFinderPlus](https://github.com/ncbi/amr) | 3.11.26 | AMR gene identification |
| [BLAST+](https://blast.ncbi.nlm.nih.gov/doc/blast-help/downloadblastdata.html)| 2.15.0 | Target gene search |
| [PlasmidFinder](https://bitbucket.org/genomicepidemiology/plasmidfinder/src/master/) | 2.1.6 | Plasmid detection |
| Cbird-Util | 1.0 | Individual summary report generation |

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4 changes: 3 additions & 1 deletion tasks/task_amrfinderplus.wdl
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Expand Up @@ -9,7 +9,7 @@ task amrfinderplus_nuc {
Float? minid
Float? mincov
Int cpu = 4
String docker = "staphb/ncbi-amrfinderplus:3.11.20-2023-09-26.1"
String docker = "staphb/ncbi-amrfinderplus:3.11.26-2023-11-15.1"
}
command <<<
# logging info
Expand Down Expand Up @@ -93,6 +93,8 @@ task amrfinderplus_nuc {
"Streptococcus agalactiae"
"Streptococcus pyogenes"
"Vibrio cholerae"
"Vibrio parahaemolyticus"
"Vibrio vulnificus"
)

# check organism in curated organism list
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2 changes: 1 addition & 1 deletion tasks/task_bbtools.wdl
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Expand Up @@ -11,7 +11,7 @@ task assembly_prep {
Int min_depth = 5
Int read_threshold = 8000000
Int memory = 8
String docker = "kincekara/bbtools:39.03"
String docker = "kincekara/bbtools:39.06"
}

command <<<
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2 changes: 1 addition & 1 deletion tasks/task_blast.wdl
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Expand Up @@ -8,7 +8,7 @@ task tblastn {
Float evalue = 0.001
Int max_hsps = 1
Int percent_identity = 90
String docker = "staphb/blast:2.14.0"
String docker = "staphb/blast:2.15.0"
}

command <<<
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2 changes: 1 addition & 1 deletion tasks/task_busco.wdl
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Expand Up @@ -4,7 +4,7 @@ task busco {
input {
File assembly
String samplename
String docker = "kincekara/busco:5.4.7-bacteria_odb10.2020-03-06"
String docker = "staphb/busco:5.6.1-prok-bacteria_odb10_2024-01-08"
Int? memory = 16
Int? cpu = 4
}
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2 changes: 1 addition & 1 deletion tasks/task_mlst.wdl
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Expand Up @@ -7,7 +7,7 @@ task ts_mlst {
input {
File assembly
String samplename
String docker = "staphb/mlst:2.23.0-2023-08"
String docker = "staphb/mlst:2.23.0-2024-01"
Int? cpu = 4
# Parameters
# --nopath Strip filename paths from FILE column (default OFF)
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2 changes: 1 addition & 1 deletion tasks/task_ncbi_datasets.wdl
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Expand Up @@ -4,7 +4,7 @@ task fetch_reference {
input {
String samplename
String taxon
String docker = "kincekara/cbird-util:alpine-v0.8"
String docker = "kincekara/cbird-util:alpine-v1.0"
}

command <<<
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2 changes: 1 addition & 1 deletion tasks/task_taxonomy.wdl
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Expand Up @@ -12,7 +12,7 @@ task profile {
String? min_hit_groups = 3
Int? memory = 32
Int? cpu = 4
String? kraken2_db_version = "Standard-8 2023-06-05"
String? kraken2_db_version = "Standard-8 2023-10-09"
}

command <<<
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2 changes: 1 addition & 1 deletion tasks/task_version.wdl
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Expand Up @@ -8,7 +8,7 @@ task version_capture {
volatile: true
}
command {
cbird_version="C-BIRD v1.3.0"
cbird_version="C-BIRD v1.4.0"
~{default='' 'export TZ=' + timezone}
date +"%Y-%m-%d" > TODAY
echo "$cbird_version" > CBIRD_VERSION
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