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Add a new example visualising brainmapper cells in specific brain reg…
…ions. (#382) * update description to reflect new brainmapper widget * Add new example plotting cells from brainmapper only in specific regions * [pre-commit.ci] auto fixes from pre-commit.com hooks for more information, see https://pre-commit.ci * Update examples/brainmapper.py Co-authored-by: Igor Tatarnikov <[email protected]> --------- Co-authored-by: pre-commit-ci[bot] <66853113+pre-commit-ci[bot]@users.noreply.github.com> Co-authored-by: Igor Tatarnikov <[email protected]>
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""" | ||
Visualise the output from brainmapper in some specific brain regions. | ||
Cells transformed to atlas space can be found at | ||
brainmapper_output/points/points.npy or exported by the brainmapper napari | ||
widget | ||
For more details on brainmapper, please see: | ||
- https://brainglobe.info/documentation/brainglobe-workflows/brainmapper/index.html | ||
- https://brainglobe.info/documentation/brainglobe-utils/transform-widget.html | ||
""" | ||
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from pathlib import Path | ||
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from myterial import orange | ||
from rich import print | ||
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from brainrender.scene import Scene | ||
from brainrender.actors import Points | ||
from brainrender import settings | ||
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import numpy as np | ||
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settings.SHADER_STYLE = "plastic" | ||
settings.SHOW_AXES = False | ||
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cells_path = Path(__file__).parent.parent / "resources" / "points.npy" | ||
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# Define regions of interest (easier to define all at the | ||
# terminal/finest level of the hierarchy) | ||
regions = ["VISp1", "VISp4", "VISp5"] | ||
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print(f"[{orange}]Running example: {Path(__file__).name}") | ||
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def get_cells_in_regions(scene, cells_path, regions): | ||
cells = np.load(cells_path) | ||
new_cells = [] | ||
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for cell in cells: | ||
if ( | ||
scene.atlas.structure_from_coords( | ||
cell, as_acronym=True, microns=True | ||
) | ||
in regions | ||
): | ||
if cell[0] > 0: | ||
new_cells.append(cell) | ||
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new_cells = np.asarray(new_cells) | ||
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return new_cells | ||
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# Create a brainrender scene | ||
scene = Scene(title=f"brainmapper cells in {regions}", inset=False) | ||
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cells_points = get_cells_in_regions(scene, cells_path, regions) | ||
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# Create points actor | ||
cells = Points(cells_points, radius=45, colors="palegoldenrod", alpha=0.8) | ||
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# Add specific regions | ||
for region in regions: | ||
scene.add_brain_region(region, color="mediumseagreen", alpha=0.2) | ||
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# Add cells | ||
scene.add(cells) | ||
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scene.render() |