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_layouts
_layouts
 
 
_sass
_sass
 
 
assets
assets
 
 
docs
docs
 
 
script
script
 
 
.gitignore
.gitignore
 
 
3D-spheroid-cell-culture.md
3D-spheroid-cell-culture.md
 
 
GST-tag-protein-purification.md
GST-tag-protein-purification.md
 
 
Gemfile
Gemfile
 
 
LICENSE
LICENSE
 
 
MBP-tag-protein-purification.md
MBP-tag-protein-purification.md
 
 
PCR-amplification.md
PCR-amplification.md
 
 
README.md
README.md
 
 
RNA-extraction-with-Trizol.md
RNA-extraction-with-Trizol.md
 
 
Siegel-Monty-oligomerization-analysis.md
Siegel-Monty-oligomerization-analysis.md
 
 
_config.yml
_config.yml
 
 
buffering-reagents.md
buffering-reagents.md
 
 
cell-cloning-by-limited-dilution.md
cell-cloning-by-limited-dilution.md
 
 
commonly-used-E-coli-genotypes.md
commonly-used-E-coli-genotypes.md
 
 
data-management.md
data-management.md
 
 
embryonic-salivary-gland-culture.md
embryonic-salivary-gland-culture.md
 
 
favicon-1.ico
favicon-1.ico
 
 
favicon.ico
favicon.ico
 
 
fluorescence-activated-cell-sorting.md
fluorescence-activated-cell-sorting.md
 
 
fluorescence-dye-labeling-of-DNA-oligos.md
fluorescence-dye-labeling-of-DNA-oligos.md
 
 
fluorescence-dye-labeling-of-proteins.md
fluorescence-dye-labeling-of-proteins.md
 
 
freezing-and-streaking-bacteria.md
freezing-and-streaking-bacteria.md
 
 
freezing-and-thawing-cells.md
freezing-and-thawing-cells.md
 
 
gel-filtration.md
gel-filtration.md
 
 
gel-purification.md
gel-purification.md
 
 
gene-knockdown-with-CasRx.md
gene-knockdown-with-CasRx.md
 
 
gene-knockout-in-C-elegans.md
gene-knockout-in-C-elegans.md
 
 
gene-knockout-in-cell-lines.md
gene-knockout-in-cell-lines.md
 
 
gene-knockout-in-mice.md
gene-knockout-in-mice.md
 
 
gibson-assembly.md
gibson-assembly.md
 
 
golden-gate-assembly.md
golden-gate-assembly.md
 
 
guide-RNA-design-CRISPRi-CRISPRa.md
guide-RNA-design-CRISPRi-CRISPRa.md
 
 
guide-RNA-design-CasRx.md
guide-RNA-design-CasRx.md
 
 
guide-RNA-design-KO.md
guide-RNA-design-KO.md
 
 
guide-RNA.md
guide-RNA.md
 
 
health_stats_Di_Shaohe.md
health_stats_Di_Shaohe.md
 
 
his-tag-protein-purification.md
his-tag-protein-purification.md
 
 
imaging-of-immunostained-cells-and-tissues.md
imaging-of-immunostained-cells-and-tissues.md
 
 
index.md
index.md
 
 
index_to_do.md
index_to_do.md
 
 
inducible-CRISPRi-and-CRISPRa.md
inducible-CRISPRi-and-CRISPRa.md
 
 
isolation-and-culture-of-SMG-epi-buds.md
isolation-and-culture-of-SMG-epi-buds.md
 
 
isolation-and-culture-of-mouse-embryonic-salivary-glands.md
isolation-and-culture-of-mouse-embryonic-salivary-glands.md
 
 
large-fragment-knockin-in-C-elegans.md
large-fragment-knockin-in-C-elegans.md
 
 
large-fragment-knockin-in-cell-lines.md
large-fragment-knockin-in-cell-lines.md
 
 
large-fragment-knockin-in-mice.md
large-fragment-knockin-in-mice.md
 
 
lentivirus-packaging.md
lentivirus-packaging.md
 
 
lentivirus-transduction-SMG-epi-buds.md
lentivirus-transduction-SMG-epi-buds.md
 
 
lentivirus-transduction-cell-lines.md
lentivirus-transduction-cell-lines.md
 
 
live-cell-imaging.md
live-cell-imaging.md
 
 
live-organ-imaging.md
live-organ-imaging.md
 
 
microtubule-co-sedimentation-worm-extracts.md
microtubule-co-sedimentation-worm-extracts.md
 
 
microtubule-co-sedimentation.md
microtubule-co-sedimentation.md
 
 
microtubule-gliding-assay.md
microtubule-gliding-assay.md
 
 
microtubule-polymerization.md
microtubule-polymerization.md
 
 
miniprep.md
miniprep.md
 
 
miraprep.md
miraprep.md
 
 
multiple-ssh-keys.md
multiple-ssh-keys.md
 
 
old-index.md
old-index.md
 
 
oxygen-scavenger.md
oxygen-scavenger.md
 
 
piggybac-mediated-transgenesis.md
piggybac-mediated-transgenesis.md
 
 
player_stats.md
player_stats.md
 
 
player_stats_header.md
player_stats_header.md
 
 
repp-assisted-plasmid-design.md
repp-assisted-plasmid-design.md
 
 
routine-cell-culture.md
routine-cell-culture.md
 
 
row-rank-equals-column-rank.md
row-rank-equals-column-rank.md
 
 
simple-e-lab-notebook.md
simple-e-lab-notebook.md
 
 
smFISH-for-tissue-culture-cells.md
smFISH-for-tissue-culture-cells.md
 
 
smFISH-for-whole-mount-mouse-embryonic-organs.md
smFISH-for-whole-mount-mouse-embryonic-organs.md
 
 
small-fragment-knockin-in-C-elegans.md
small-fragment-knockin-in-C-elegans.md
 
 
small-fragment-knockin-in-cell-lines.md
small-fragment-knockin-in-cell-lines.md
 
 
small-fragment-knockin-in-mice.md
small-fragment-knockin-in-mice.md
 
 
sucrose-gradient.md
sucrose-gradient.md
 
 
thumbnail.png
thumbnail.png
 
 
tissue-dissociation-into-single-cells.md
tissue-dissociation-into-single-cells.md
 
 
useful-cell-numbers-in-various-culture-formats.md
useful-cell-numbers-in-various-culture-formats.md
 
 
whole-mount-immunostaining-embryonic-organs-or-spheroids.md
whole-mount-immunostaining-embryonic-organs-or-spheroids.md
 
 

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This is snownontrace Github pages

This website hosts useful protocols for biological benchwork and data analysis. Benchwork protocols cover molecular cloning, biochemistry, microscopy, cell culture, lentivirus, genome engineering and more. Data analysis focus on image and sequence analysis.

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